Antioxidant Activity of Two Flavonol Glycosides from Cirsium Hypoleucum Dc. through Bioassay- Guided Fractionation
نویسندگان
چکیده
The flowers, roots and stems of Cirsium species are used as a folk remedy in Anatolia. In order to evaluate antioxidant activity of Cirsium hypoleucum which is an endemic plant, superoxide radical (hypoxanthine-xanthine oxidase system) and free radical scavenging activities (DPPH) using in vitro electron spin resonance (ESR) spectrometry of the different extracts, fractions and compounds obtained by Bioassay Guided Fractionation (BAGF) technique were studied. The bioassay guided fractionation of methanol extract led to the isolation flavonoid 1 and 2 from n–butanol fr. The structures of these compounds were elucidated using UV, IR, MS, Hand C-NMR techniques as quercetin-3-O-rutinoside (rutin) (1) and kaempferol-3-O-rutinoside (nicotiflorin) (2). Flavonoid 1 showed more potent antioxidant activity than flavonoid 2.
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